CUG team led by Prof. XIA Fan from the School of Materials Science and Chemistry published “Spatiotemporal patterning of photoresponsive DNA-based hydrogels to tune local cell responses” in Nature Communications on April 22, 2021. The first author is Prof. HUANG Fujian from the School of Materials Science, and the corresponding authors are Prof. HUANG Fujian, Prof. XIA Fan, and Prof. Itamar Willne from the Hebrew University of Jerusalem. The abstract of the paper is as follows.
Understanding the spatiotemporal effects of surface topographies and modulated stiffness and anisotropic stresses of hydrogels on cell growth remains a biophysical challenge. Here we introduce the photolithographic patterning or two-photon laser scanning confocal microscopy patterning of a series of o-nitrobenzylphosphate ester nucleic acid-based polyacrylamide hydrogel films generating periodically-spaced circular patterned domains surrounded by continuous hydrogel matrices. The patterning processes lead to guided modulated stiffness differences between the patterned domains and the surrounding hydrogel matrices, and to the selective functionalization of sub-regions of the films with nucleic acid anchoring tethers. HeLa cells are deposited on the circularly-shaped domains functionalized with the MUC-1 aptamers. Initiation of the hybridization chain reaction by nucleic acid tethers associated with the continuous hydrogel matrix results in stress-induced ordered orthogonal shape-changes on the patterned domains, leading to ordered shapes of cell aggregates bound to the patterns.
Photopatterning of circular domains functionalized with the toehold activated duplexes in the continuous (4)-crosslinked hydrogel matrix modified with hairpins (3).
